What is Giemsa stain made of?
Giemsa’s solution is a mixture of methylene blue, eosin, and Azure B. The stain is usually prepared from commercially available Giemsa powder. A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide.
How do you make a 10% Giemsa stain?
Make up a 10% Giemsa solution with distilled/deionized water buffered to pH 7.2. If only one slide is to be stained, you will require about 3 ml of prepared stain. Allow 3 drops of stock Giemsa solution (from the Pasteur pipette) to each millilitre of buffered water to give a 10% solution.
Is Giemsa stain acidic or basic?
Principle of Giemsa Stain Azure and eosin are acidic dye that variably stains the basic components of the cells like the cytoplasm, granules etc. Methylene blue acts as the basic dye, which stains the acidic components, especially the nucleus of the cell.
What is the use of giemsa?
Giemsa stain is a gold standard staining technique that is used for both thin and thick smears to examine blood for malaria parasites, a routine check-up for other blood parasites and to morphologically differentiate the nuclear and cytoplasm of Erythrocytes, leucocytes and Platelets and parasites.
How do you make a 5% Giemsa solution?
Popular Answers (1)
- Dissolve 3.8g of Giemsa powder into 250ml of methanol.
- Heat the solution from step 1 to ~60oC.
- Slowly add in 250ml of glycerin to the solution from step 2.
- Filter the solution from step 3.
- The solution needs to stand a period of time prior to use.
What is the difference between Giemsa and Leishman stain?
The key difference between Giemsa Stain and Leishman Stain is that Giemsa staining is useful in the staining of DNA regions of different chromosomes to investigate different aberrations such as translocations and rearrangements, while Leishman stain is useful during blood smear staining and analysis to differentiate …
How long is gene?
In humans, genes vary in size from a few hundred DNA bases to more than 2 million bases. An international research effort called the Human Genome Project, which worked to determine the sequence of the human genome and identify the genes that it contains, estimated that humans have between 20,000 and 25,000 genes.
How do you make a 10% Giemsa?
Is Leishman a romanowsky stain?
Stains that are related to or derived from the Romanowsky-type stains include Giemsa, Jenner, Wright, Field, May–Grünwald and Leishman stains.
Why is a thin smear better than a thick smear?
1. Thick blood smears are most useful for detecting the presence of parasites. Thin blood smears helps to discover which species of parasite is causing the infection.